HDStIM: High Dimensional Stimulation Immune Mapping ('HDStIM')
A method for identifying responses to experimental stimulation in mass or flow cytometry that uses high dimensional analysis of measured parameters and can be performed with an end-to-end unsupervised approach. In the context of in vitro stimulation assays where high-parameter cytometry was used to monitor intracellular response markers, using cell populations annotated either through automated clustering or manual gating for a combined set of stimulated and unstimulated samples, 'HDStIM' labels cells as responding or non-responding. The package also provides auxiliary functions to rank intracellular markers based on their contribution to identifying responses and generating diagnostic plots.
||R (≥ 3.6.0)
||tibble, ggplot2, uwot, dplyr, tidyr, broom, tidyselect, ggridges, Boruta, scales
||knitr, rmarkdown, testthat
John Tsang [aut,
||Rohit Farmer <rohit.farmer at gmail.com>
||CC0 | file LICENSE
Please use the canonical form
to link to this page.